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The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR merchandise have been digested and cloned into a pET21a+ vector employing NdeI and XhoI restriction enzymes. A degree mutation was launched by the short alter site-directed mutagenesis tec
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